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|Hela Lysate||3||= 2.95%|
|Hela Lysate||3||= 7.25%|
Abcam’s Monoamine Oxidase Type B (MAOB) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of MAOB protein in human cell and tissue lysates.
The assay employs an antibody specific to MAOB protein coated onto well plate strips. Controls and samples are pipetted into the wells and MAOB present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti-MAOB detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of MAOB bound to the plate well. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Monoamine Oxidase Type B (MAOB, Amine oxidase [flavin-containing] B, P27338) is a 58 kDa enzyme that belongs to the flavin monoamine oxidase family. Two subtypes of Monoamine Oxidase have been identified: MAO-A and MAO-B. MAOB is an enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine, phenylethylamine, and dopamine (DA), while MAOA prefers to metabolize norepinephrine (NE), serotonin (5-HT), and dopamine (DA).
The differences between the substrate selectivity of the two enzymes are utilized clinically when treating specific disorders: MAOA inhibitors have been used in the treatment of depression, and MAOB inhibitors are used in the treatment of Parkinson's disease.
|Components||1 x 96 tests|
|10X Blocking Buffer||1 x 6ml|
|10X HRP Label||1 x 1ml|
|10X MAOB Detector Antibody||1 x 1ml|
|20X Buffer||1 x 20ml|
|Extraction Buffer||1 x 15ml|
|HLH Lysate Control||1 x 200µg|
|HRP Development Solution||1 x 12ml|
|MAOB Microplate||1 unit|
Our Abpromise guarantee covers the use of ab157393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
The specificity of the MAOB Capture Antibody used in this ELISA kit was demonstrated by MAOB activity assay using ab109912 Monoamine oxidase B (MAOB) Specific Activity Assay Kit with MAOB specific inhibitor, Pargyline, to show the isozyme specificity. The Antibody also immunoprecipitated MAOB protein from multiple samples, the immunocaptured target has been confirmed by Mass spectrometric data.
ab157393 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"