The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Monocarboxylate transporter 1
Monocarboxylate transporter isoform 1
Monocarboxylic acid transporter 1
Solute carrier family 16 (monocarboxylic acid transporters) member 1
Solute carrier family 16 member 1
Solute carrier family 16 member 1 (monocarboxylic acid transporter 1)
Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate.
Widely expressed in normal and in cancer cells.
Involvement in disease
Symptomatic deficiency in lactate transport Familial hyperinsulinemic hypoglycemia 7
Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.