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|Sample type||Average %||Range|
|Serum||89||86% - 93%|
|Cell culture media||108||99% - 123%|
mtTFA in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mtTFA protein in human cell and tissue extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
mtTFA (mitochondrial transcription factor A or TFAM) is a nuclear DNA encoded mitochondrial protein involved with mitochondrial DNA transcription and packaging. mtTFA binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. mtTFA is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. mtTFA is required for the maintenance of normal levels of mitochondrial DNA and may play a role in organizing and compacting mitochondrial DNA. mtTFA is able to unwind and bend DNA.
|Components||1 x 96 tests|
|10X mtTFA Capture Antibody||1 x 600µl|
|10X mtTFA Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|50X Cell Extraction Enhancer Solution (ab193971)||1 x 1ml|
|5X Cell Extraction Buffer PTR||1 x 10ml|
|Antibody Diluent 5BI||1 x 6ml|
|mtTFA Human Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS (ab193972)||1 x 12ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 x 96 tests|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab181425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
Background subtracted data is plotted for the indicated Human samples (average of duplicate values plotted at each data point).
Interpolated values (corrected for dilution factor relative to 50 µg/mL) are shown for the indicated loads of HeLa, HL-60 and HepG2 cell extracts. For each cell line, comparable results are attained for all concentrations of extract load (CV < 6% for all determinations within a cell line).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"