Abcam’s anti-Mumps virus IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Mumps virus in Human serum and plasma.
A 96-well plate has been precoated with Mumps virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Mumps virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mumps virus IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 unit|
|IgM Sample Diluent||white cap||1 x 100ml|
|Mumps virus (IgM) Coated Microplate (12 x 8 wells)||12 breakapart 8-well snap-off strips||1 unit|
|Mumps virus anti-IgM HRP Conjugate||black cap||1 x 20ml|
|Mumps virus IgM Cut-off Control||green cap||1 x 3ml|
|Mumps virus IgM Negative Control||blue cap||1 x 2ml|
|Mumps virus IgM Positive Control||red cap||1 x 2ml|
|Stop Solution||1 x 15ml|
|TMB Substrate Solution||Yellow cap||1 unit|
Our Abpromise guarantee covers the use of ab108753 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108753 has not yet been referenced specifically in any publications.