Human Anti-Mycoplasma pneumoniae IgA ELISA Kit (ab108754)

Overview

  • Product nameHuman Anti-Mycoplasma pneumoniae IgA ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Neg. Serum 20 6.1%
    Pos. Serum 24 3.5%
    Inter-assay
    Sample n Mean SD CV%
    Neg. Serum 12 8.1%
    Pos. Serum 12 3.9%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Mycoplasma pneumoniae IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Mycoplasma pneumoniae in Human serum and plasma.

     

    A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgA sample captured in plate.

     

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108754 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent concentration.

    Protocols

    References for Human Anti-Mycoplasma pneumoniae IgA ELISA Kit (ab108754)

    ab108754 has not yet been referenced specifically in any publications.

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