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Abcam’s anti-Mycoplasma pneumoniae IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Mycoplasma pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgA sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|IgA Sample Diluent||white cap||1 x 100ml|
|Mycoplasma pneumoniae (IgA) Coated Microplate (12 x 8 wells)||12 breakapart 8-well snap-off strips||1 unit|
|Mycoplasma pneumoniae anti-IgA HRP Conjugate||black cap||1 x 20ml|
|Mycoplasma pneumoniae IgA Cut-off Control||green cap||1 x 3ml|
|Mycoplasma pneumoniae IgA Negative Control||blue cap||1 x 2ml|
|Mycoplasma pneumoniae IgA Positive Control||red cap||1 x 2ml|
|Stop Solution||1 x 15ml|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108754 has not yet been referenced specifically in any publications.