Abcam’s anti-Mycoplasma pneumoniae IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Mycoplasma pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgG sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|IgG Sample Diluent||white cap||1 x 100ml|
|Mycoplasma pneumoniae (IgG) Coated Microplate (12 x 8 wells)||12 breakapart 8-well snap-off strips||1 unit|
|Mycoplasma pneumoniae anti-IgG HRP Conjugate||black cap||1 x 20ml|
|Mycoplasma pneumoniae IgG Cut-off Control||green cap||1 x 3ml|
|Mycoplasma pneumoniae IgG Negative Control||blue cap||1 x 2ml|
|Mycoplasma pneumoniae IgG Positive Control||red cap||1 x 2ml|
|Stop Solution||1 x 15ml|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab108755 has not yet been referenced specifically in any publications.