Abcam’s anti-Mycoplasma pneumoniae IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Mycoplasma pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgM sample captured in plate.
|Components||Identifier||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgM Sample Diluent||Green, White cap||1 x 100ml|
|Mycoplasma pneumoniae (IgM) Coated Microplate (12 x 8 wells)||12 x 8 Well Strips||1 unit|
|Mycoplasma pneumoniae anti-IgM HRP Conjugate||Red, Black cap||1 x 20ml|
|Mycoplasma pneumoniae IgM Cut-off Control||Yellow, Green cap||1 x 3ml|
|Mycoplasma pneumoniae IgM Negative Control||Yellow, Blue cap||1 x 2ml|
|Mycoplasma pneumoniae IgM Positive Control||Yellow, Red cap||1 x 2ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
Our Abpromise guarantee covers the use of ab108756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108756 has not yet been referenced specifically in any publications.