Overview

  • Product nameHuman Myoglobin ELISA Kit
    See all Myoglobin kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 5 ng/ml
  • Range
    5 ng/ml - 1000 ng/ml
  • Recovery

    102.8 %

    Sample specific recovery
    Sample type Average % Range
    Serum 102.8 39ng/ml - 780ng/ml

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Myoglobin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myoglobin in Human serum.

    A 96-well plate has been precoated with anti-Myoglobin antibodies. Samples and standards and are added to the wells, where Myoglobin in the sample and standards binds to the precoated antibody. Added anti-Myoglobin HRP conjugate binds to this antibody-Myoglobin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Myoglobin in the sample and the intensity is measured at 450 nm.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Myoglobin ELISA Kit images

  • Representative standard curve using ab108652

Protocols

References for Human Myoglobin ELISA Kit (ab108652)

This product has been referenced in:
  • Naclerio F  et al. A multi-ingredient containing carbohydrate, proteins L-glutamine and L-carnitine attenuates fatigue perception with no effect on performance, muscle damage or immunity in soccer players. PLoS One 10:e0125188 (2015). Human . Read more (PubMed: 25915424) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. The kit is for one 96 well plate, so taking into account 5 standards and a blank, plus doing everything in duplicate allows you to run 42 unique samples.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"