|Sample type||Average %||Range|
|Serum||102.8||39ng/ml - 780ng/ml|
Abcam’s Myoglobin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myoglobin in Human serum.
A 96-well plate has been precoated with anti-Myoglobin antibodies. Samples and standards and are added to the wells, where Myoglobin in the sample and standards binds to the precoated antibody. Added anti-Myoglobin HRP conjugate binds to this antibody-Myoglobin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Myoglobin in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Myoglobin Coated Microplate (12 x 8 wells)||1 unit|
|Anti-Myoglobin HRP Conjugate||1 x 22ml|
|Myoglobin Standard 0 - 0 ng/mL||1 x 0.5ml|
|Myoglobin Standard 1 - 25 ng/mL||1 x 0.5ml|
|Myoglobin Standard 2 - 100 ng/mL||1 x 0.5ml|
|Myoglobin Standard 3 - 250 ng/mL||1 x 0.5ml|
|Myoglobin Standard 4 - 500 ng/mL||1 x 0.5ml|
|Myoglobin Standard 5 - 1,000 ng/mL||1 x 0.5ml|
|Sample Diluent||1 x 25ml|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab108652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|