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|Sample type||Average %||Range|
|Serum||103||100% - 108%|
|Cell culture media||94||91% - 97%|
|Heparin Plasma||95||92% - 97%|
|EDTA Plasma||83||77% - 91%|
|Citrate Plasma||92||82% - 98%|
Quantify up to 75 proteins and peptides per well, using just 12.5 µL of sample, with FirePlex® immunoassays. The assay takes approximately 3.5 hours, followed by particle analysis using a compatible flow cytometer and data analysis using our free FirePlex Analysis Workbench software.
Benefits of the assay
- Quantify both low and high abundance analytes with a typical sensitivity of 0.5 pg /mL and a fully extended dynamic range spanning 4.5 logs
- Choose from hundreds of analytes to easily build your own custom panel, use a convenient pre-mixed pre-designed panel, or add new analytes to a pre-designed panel
- Consistent batch-to-batch performance with assays built using the same recombinant monoclonal antibody pairs as our SimpleStep ELISA® and Matched Antibody Pair Kits
- One FirePlex particle platform for both multiplex miRNA assays and multiplex immunoassays
Find out more about multiplex immunoassays using FirePlex particles here
Important - Use of the FirePlex Multiplex Immunoassay Kit also requires the purchase of:
- a) Human Multiplex Immunoassay Core Kit, ab208203; and
- b) Multiplex Immunoassay Protein Standard Mix, such as ab219000.
FirePlex® is a registered trademark in the United States and is an unregistered trademark elsewhere.
The following information provides the sensitivity range and recommended starting dilutions for validated biological samples.
Sensitivity range - 28.9 - 55.8 pg/mL
|Recommended Dilution Range|
|Components||Identifier||1 x 96 tests|
|75X Human Myoglobin Capture Particles||Particle Code - 09||1 unit|
|Human Myoglobin Biotin Detector (Lyophilized)||1 unit|
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 11.9 ng/mL with a range of 2.9 – 26.3 ng/mL.
The concentrations of Myoglobin were interpolated from the Myoglobin standard curves and corrected for sample dilution. 1X diluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (heparin) 25% and plasma (citrate) 25%. The interpolated dilution factor corrected mean values are plotted. The mean Myoglobin concentration was determined to be 18.1 ng/mL in serum, 73.0 ng/mL in plasma (EDTA), 17.1 ng/mL in plasma (heparin) and 21.7 ng/mL in plasma (citrate).
The concentrations of Myoglobin were interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected mean values are plotted. The mean Myoglobin concentration was determined to be 66126 pg/mL in cell culture media containing 10% fetal bovine serum.
The concentrations of Myoglobin were interpolated from the Myoglobin standard curves and corrected for sample dilution. 1X diluted samples are as follows: 25% urine, cerebrospinal fluid (CSF) and bronchial lavage. The interpolated dilution factor corrected values are plotted. The mean Myoglobin concentration was determined to be 797 pg/mL in urine, 638 pg/mL in CSF and 45.4 pg/mL in bronchial lavage.
ab219032 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"