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Abcam’s Human NCAM1 (CD56) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human NCAM1 (CD56) in cell culture supernatants, serum and plasma (heparin, EDTA).
A NCAM1 (CD56) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for NCAM1 (CD56) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human NCAM1 (CD56) amount of sample captured in plate.
|Components||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-Human NCAM1 antibody Microplate (12 x 8 wells)||1 unit|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated anti- Human NCAM1 antibody||1 x 130µl|
|Lyophilized recombinant Human NCAM1 standard||2 x 10ng|
|Plate Seal||4 units|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab119587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119587 has not yet been referenced specifically in any publications.