Overview

  • Product name
    Human Neurotrophin 3 ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 4 pg/ml
  • Range
    4.12 pg/ml - 3000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.25 87% - 105%
    Serum 95 85% - 103%
    Plasma 94.45 84% - 105%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Neurotrophin 3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 3 in serum, plasma, and cell culture supernatants. (Human Neurotrophin 3 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human Neurotrophin 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • NT-3 measured in biologicals showing quantity (pg) per mL tested sample

  • Representative Standard Curve using ab100615.

  • Representative Standard Curve using ab100615.

Protocols

References

ab100615 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam. Please find attached the Certificate of Compliance for ab100615 as requested. If there is anything else I can help you with, please let me kn

Thank you for your inquiry. We haven't tested ab100615 with cell lysates.  It would most likely work, but we wouldn't be able to guarantee it since it is untested.   If you decide to test this kit with lysate samples, we recommend diluting the samp...

Read More

Thank you very much for your interest in ab100615. This kit has not been validated for use with mouse samples, but is predicted to react based on high sequence homology. I can offer a discount off a future purchase if you buy either this ELI...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up