The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-NIR2 antibody (ab22823)
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Drosophila retinal degeneration B
Drosophila retinal degeneration B homolog
Membrane-associated phosphatidylinositol transfer protein 1
Phosphatidylinositol transfer protein
phosphatidylinositol transfer protein membrane associated
PYK2 N terminal domain interacting receptor 2
Pyk2 N-terminal domain-interacting receptor 2
retinal degeneration B alpha 1
FunctionRegulates RHOA activity, and plays a role in cytoskeleton remodeling. Necessary for normal completion of cytokinesis. Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus. Binds phosphatidyl inositol phosphates (in vitro). May catalyze the transfer of phosphatidylinositol and phosphatidylcholine between membranes (By similarity). Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus. Required for protein export from the endoplasmic reticulum and the Golgi. Binds calcium ions.
Sequence similaritiesBelongs to the PtdIns transfer protein family. PI transfer class IIA subfamily. Contains 1 DDHD domain.
Post-translational modificationsPhosphorylated on multiple sites by CDK1 at the onset of mitosis. Phosphorylation facilitates dissociation from the Golgi complex and is required for interaction with PLK1. Phosphorylated on threonine residues upon treatment with oleic acid. Phosphorylated on tyrosine residues by PTK2B.
Cellular localizationCytoplasm. Golgi apparatus > Golgi stack membrane. Endoplasmic reticulum membrane. Lipid droplet. Cleavage furrow. Midbody. Peripheral membrane protein associated with Golgi stacks in interphase cells. A minor proportion is associated with the endoplasmic reticulum. Associated with lipid droplets. Dissociates from the Golgi early on in mitosis and localizes to the cleavage furrow and midbody during cytokinesis.