Overview

  • Product name
    Human Notch-1 ELISA Kit (TAN-1)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    0.029 ng/ml - 7 ng/ml
  • Recovery

    > 86 %

    Sample specific recovery
    Sample type Average % Range
    Serum = 96.06 88% - 102%
    Plasma = 93.78 86% - 102%
    Cell culture media = 103.1 93% - 113%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Notch-1 (TAN-1) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Notch-1 (TAN-1) in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human Notch-1(TAN-1) coated on a 96-well plate. Standards and samples are pipetted into the wells and Notch-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated Anti-Human Notch-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Notch-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

    This kit can detect extracellular domain of NOTCH1.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155437 - Assay Diluent B.

  • Representative standard curve using ab155437 -  Assay Diluent A.

Protocols

References

ab155437 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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