|Sample type||Average %||Range|
|Cell culture supernatant||114.9||102% - 123%|
|Serum||97.2||86% - 107%|
|Plasma||86.02||79% - 94%|
Abcam’s Human NRG1 beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRG1 beta 1 in serum, plasma, and cell culture supernatants. (Collect plasma using EDTA or citrate as an anticoagulant. Heparinized plasma is not recommended. Human NRG1 beta 1 concentration is pretty low in normal serum/plasma, it may not be detected in this assay)
This assay employs an antibody specific for Human NRG1 beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NRG1 beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NRG1 beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human NRG1 beta 1||2 vials|
|NRG1 beta 1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human NRG1 beta 1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100614 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
NRG1 beta measured in culture supernatants, THP-1 levels below level of detection (duplicates +/- SD).
NRG1 beta measured in human biological fluids; signals from serum and from rat and mouse samples were below level of detection (duplicates +/- SD).