ab191181 recognizes an antigen associated with the nuclei in human cells.
It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. It can be used with paraformaldehyde-fixed frozen tissue or cell preparations.
Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Primary antibody was used at 1ug/ml for 60 minutes at room temp. AF647 conjugated goat anti-mouse IgG was used as the secondary antibody (red). Cells were counterstained with AF488 conjugated Phalloidin (green).
Anti-Human Nuclear Antigen antibody [235-1] ab191181 Frozen Human Tonsil fixed in cold acetone, Primary Ab used at 0.5ug/ml for 30 minutes at room temp. Visualised using a HRP-Polymer detection system.
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