Overview

  • Product nameHuman Nucleolin peptide

Description

  • NatureSynthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab25315 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Nucleolin antibody (ab22758)

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • C23
    • FLJ45706
    • MS1116
    • NCL
    • Nucl
    • NUCL_HUMAN
    • Nucleolin
    • Protein C23
    see all
  • FunctionNucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
  • Sequence similaritiesContains 4 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • Cellular localizationNucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt

References for Human Nucleolin peptide (ab25315)

This product has been referenced in:
  • Li Z  et al. Aptamer-Capped Multifunctional Mesoporous Strontium Hydroxyapatite Nanovehicle for Cancer-Cell-Responsive Drug Delivery and Imaging. Biomacromolecules : (2012). Read more (PubMed: 23140615) »
  • Fabricius EM  et al. Immunohistochemical determination of the appropriate anti-hTERT antibodies for in situ detection of telomerase activity in frozen sections of head and neck squamous cell carcinomas and tumor margin tissues. Int J Oncol 34:1257-79 (2009). Blocking . Read more (PubMed: 19360339) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

Although the antibody from Santa Cruz, MS-3 was raised against the full length protein, because it is a monoclonal antibody it will only recognize a single epitope on the protein. There is a very good chance that the...

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Thank you for your reply.

I am not sure what catalog number you mean by "MS3" but if the antibody is a polyclonal raised against the full length protein it is likely to show weak reactivity with the peptide ab25315 because only a fraction of ...

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The peptide ab25315 is a 14 amino acid peptide from within residues 1-100 of human Nucleolin. The exact sequence is considered to be proprietary because this peptide was used as an immunogen for ab22758. The 706 a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"