Overview

  • Product name
    Human Oncostatin M ELISA Kit
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    1.37 pg/ml - 1000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 126.2 117% - 136%
    Plasma 76.63 83% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Oncostatin M Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Oncostatin M in plasma and cell culture supernatants. (GeneCards: Osm) 


    Note: Human Oncostatin M concentration is pretty low in normal plasma, it may not be detected in this assay.


    We have not been able to detect endogenous Human Oncostatin M in normal serum with ab100619, only in serum spiked with Human Oncostatin M.


    This assay employs an antibody specific for Human Oncostatin M coated on a 96- well plate. Standards and samples are pipetted into the wells and Oncostatin M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Oncostatin M antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Oncostatin M bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


     

  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100619.

Protocols

References

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). Read more (PubMed: 26732624) »
  • Hollmén M  et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). Human . Read more (PubMed: 27141367) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. I am sorry to confirm that ab100619 Oncostatin M Human ELISA Kit has only been validated for serum, pasma, cell culture spernatants, and has not been tested with CSF.  However, this ELISA kit should be accommodating to a ...

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It is very difficult for us to recommend dilutions for kits like this since samples can have a huge range in the concentration of protein present depending on the samples. The best thing to do to is to run a representative samples in a serial dilution ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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