Overview

  • Product nameHuman Oncostatin M ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    1.37 pg/ml - 1000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 126.2 117% - 136%
    Serum 89.86 80% - 99%
    Plasma 76.63 83% - 102%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Oncostatin M Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Oncostatin M in serum, plasma, and cell culture supernatants. (Human Oncostatin M concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

    This assay employs an antibody specific for Human Oncostatin M coated on a 96- well plate. Standards and samples are pipetted into the wells and Oncostatin M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Oncostatin M antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Oncostatin M bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionGrowth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
  • Sequence similaritiesBelongs to the LIF/OSM family.
  • Post-translational
    modifications
    Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • MGC20461
    • ONCM_HUMAN
    • Oncostatin M precursor
    • Oncostatin-M
    • OncostatinM
    • OSM
    • OTTMUSP00000005249
    • RP23-453B22.1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Oncostatin M ELISA Kit images

  • Representative Standard Curve using ab100619.

Protocols

References for Human Oncostatin M ELISA Kit (ab100619)

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). Read more (PubMed: 26732624) »
  • Croci DO  et al. Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma. J Exp Med 209:1985-2000 (2012). Human . Read more (PubMed: 23027923) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. I am sorry to confirm that ab100619 Oncostatin M Human ELISA Kit has only been validated for serum, pasma, cell culture spernatants, and has not been tested with CSF.  However, this ELISA kit should be accommodating to a ...

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It is very difficult for us to recommend dilutions for kits like this since samples can have a huge range in the concentration of protein present depending on the samples. The best thing to do to is to run a representative samples in a serial dilution ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"