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|Sample type||Average %||Range|
|Cell culture supernatant||126.2||117% - 136%|
|Plasma||76.63||83% - 102%|
Abcam’s Oncostatin M Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Oncostatin M in plasma and cell culture supernatants. (Human Oncostatin M concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Oncostatin M coated on a 96- well plate. Standards and samples are pipetted into the wells and Oncostatin M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Oncostatin M antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Oncostatin M bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human Oncostatin M in normal serum with ab100619, only in serum spiked with Human Oncostatin M.
Optimization may be required with urine samples
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-Human Oncostatin M||2 vials|
|Oncostatin M Microplate (12 x 8 wells)||1 unit|
|Recombinant Human Oncostatin M Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100619 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|