• Product nameHuman Osteopontin ELISA Kit
    See all Osteopontin kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Urine, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 50 pg/ml
  • Range
    74.07 pg/ml - 18000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103.5 95% - 118%
    Serum 89.92 88% - 108%
    Plasma 92.41 90% - 109%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Osteopontin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteopontin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionBinds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificityBone. Found in plasma.
  • Sequence similaritiesBelongs to the osteopontin family.
  • Post-translational
    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • BNSP
    • Bone sialoprotein 1
    • BSP I
    • BSPI
    • Early T lymphocyte activation 1
    • ETA 1
    • ETA1
    • MGC110940
    • Nephropontin
    • OPN
    • Osteopontin
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
    • PSEC0156
    • Secreted phosphoprotein 1
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
    • SPP 1
    • SPP-1
    • SPP1
    • SPP1/CALPHA1 fusion
    • Urinary stone protein
    • Uropontin
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Osteopontin ELISA Kit images

  • Osteopontin measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum diluted 10-30 fold. Human plasma diluted 50 fold - below detection. Human milk diluted 18000-54000 fold. Human urine diluted 100 fold.

  • Representative Standard Curve using ab100618.

  • Representative Standard Curve using ab100618.


References for Human Osteopontin ELISA Kit (ab100618)

This product has been referenced in:
  • Arner E  et al. Ceruloplasmin is a novel adipokine which is overexpressed in adipose tissue of obese subjects and in obesity-associated cancer cells. PLoS One 9:e80274 (2014). Read more (PubMed: 24676332) »
  • Reza S  et al. Expression of osteopontin in patients with thyroid dysfunction. PLoS One 8:e56533 (2013). ELISA ; Human . Read more (PubMed: 23437160) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.
Though we have not tested the protein ab81549 (http://www.abcam.com/osteopontin-protein-active-ab81549.html) with ab100618. However it is most likely to work.
I hope this information is helpful to you. Please do no...

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Thank you for taking the time to contact me and let us know.

I'm pleased to hear the results were successful and that you have found a suitable dilution factor for your samples.

Good luck with your research, and please do not hesi...

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Thank you for your enquiry.

I can confirm that the suggested dilution for normal serum/plasma when testing with this kit is 3-100 fold.

Please note that this will depend on expression level in the samples you have and so this is a...

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Thank youfor your inquiry.

The quote for ab8448 has been send in a separate email.

I am happy to confirm that we offer ELISA kits for Osteopontin:

ab100618 (Osteopontin Human ELISA Kit)


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Thank you for contacting us.

We received the following explanation from the lab:

The full length Osteopontin protein (aa 17-314) was used as the immunogen for the antibodies used in thekit ab100618. And as epitope mapping was not p...

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Lamento mucho lo ocurrido. Es más que probable que los resultados obtenidos no sean correctos debido a la contaminación del buffer.

Os he mandado un kit nuevo completamente gratuito para compensar. Recibiréis un mail auto...

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Thank you for contacting Abcam. I am sorry that you did not receive the full amount of product and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact ...

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Thank you for your patience. Here is the lab's response to your question: While testing fresh media is always ideal, frozen media is usually more practical and will certainly be compatible with our ELISA kits. I hope this information is helpf...

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