Human OTUB1 protein fragment (ab91823)
- Product nameHuman OTUB1 protein fragmentSee all OTUB1 proteins and peptides ...
- SourceE. coli
- Amino Acid Sequence
- SequenceMAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQE IAVQNPLVSERLEL SVLYKEYAEDDNIYQQKIKDLHKK YSYIRKTRPDGNCFYRAFGFSHLEALLDDS KELQRFKA VSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVA DLLASF NDQ
- Amino acids1 to 165
Our Abpromise guarantee covers the use of ab91823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Additional NotesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
- Concentration information loading...
Preparation and Storage
- Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
- ReconstitutionReconstitute with 125 µl aqua dest.
- Deubiquitinating enzyme OTUB1
- OTU domain containing ubiquitin aldehyde binding protein 1
- OTU domain, ubiquitin aldehyde binding 1
- OTU domain-containing Ubal-binding protein 1
- OTU domain-containing ubiquitin aldehyde-binding protein 1
- OTU-domain Ubal-binding 1
- Otubain 1
- Ubiquitin specific processing protease OTUB1
- Ubiquitin thioesterase OTUB1
- ubiquitin-specific protease otubain 1
- Ubiquitin-specific-processing protease OTUB1
- FunctionHydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform 1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform 2 stabilizes RNF128 and promotes anergy. Surprisingly, it regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). Mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. Not able to cleave di-ubiquitin. Also capable of removing NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to 'Lys-48'-linked ubiquitin.
- Tissue specificityIsoform 1 is ubiquitous. Isoform 2 is expressed only in lymphoid tissues such as tonsils, lymph nodes and spleen, as well as peripheral blood mononuclear cells.
- Sequence similaritiesBelongs to the peptidase C65 family.
Contains 1 OTU domain.
- DomainIn addition to ubiquitin-binding at the Cys-91 active site, a proximal ubiquitin-binding site is also present at Cys-23 Occupancy of the active site is needed to enable tight binding to the second site. Distinct binding sites for the ubiquitins may allow to discriminate among different isopeptide linkages (i.e. 'Lys-48'-, 'Lys-63'-linked polyubiquitin) in polyubiquitin substrates and achieve linkage-specific deubiquitination.
- Cellular localizationCytoplasm.
Human OTUB1 protein fragment images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for Human OTUB1 protein fragment (ab91823)
ab91823 has not yet been referenced specifically in any publications.