Overview

  • Product nameHuman PABP peptide

Description

  • NatureSynthetic
  • Amino Acid Sequence
    • AccessionP11940
    • SpeciesHuman

Associated products

Specifications

Our Abpromise guarantee covers the use of ab23710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • PAB 1
    • PAB1
    • PABP 1
    • PABP1
    • PABPC 1
    • PABPC1
    • PABPC2
    • PABPL1
    • Poly A binding protein 1
    • Poly A binding protein cytoplasmic 1
    • poly(A) binding protein, cytoplasmic 1
    • poly(A) binding protein, cytoplasmic 2
    • Polyadenylate binding protein 1
    see all
  • RelevanceThe poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. Grange et al. (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633-amino acid protein contains 4 repeats of an approximately 80-amino acid unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73 kD by SDS-PAGE. Northern blot analysis indicated that PABP is expressed as a 2.9-kb mRNA in human melanoma cells. Gorlach et al. (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 10.3 and is a very abundant, stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However, using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP), Afonina et al. (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited, suggesting that active transcription is required for nuclear export of PABP1.
  • Cellular localizationCytoplasmic. Shuttles between the cytoplasm and the nucleus.

References for Human PABP peptide (ab23710)

ab23710 has not yet been referenced specifically in any publications.

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The peptide ab23710 is only 13 a.a. long. The listing of the "Protein length" on the datasheet is how long the full length protein is according to Swiss Prot (636 a.a. long).

http://www.uniprot....

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There is not a specific concentration recommended for the use of this antibody in ELISA. Optimisation is required with different dilutions in order to assess the best one for your particular conditions. We can how...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"