Overview

  • Product nameHuman PAI1 ELISA Kit (SERPINE1)
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.8%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue, Cerebral Spinal Fluid
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.08 ng/ml
  • Range
    0.078 ng/ml - 5 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PAI1 (SERPINE1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.

    A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.

    This assay recognizes both natural and recombinant Human PAI-1.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionSerine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificityFound in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in diseaseDefects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similaritiesBelongs to the serpin family.
  • Post-translational
    modifications
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • PAI1_HUMAN
    • Plasminogen activator inhibitor 1
    • Serpin E1
    • SERPINE1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human PAI1 ELISA Kit (SERPINE1) images

  • Standard curve mean of duplicates (+/- SD) with background readings subtracted

  • PAI1 measured in various samples showing quantity (ng) per mL of tested sample

  • PAI1 measured in cell lysates showing quantity (ng) per 1 million cells tested

Protocols

References for Human PAI1 ELISA Kit (SERPINE1) (ab108891)

This product has been referenced in:
  • Giacoia EG  et al. PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation. Mol Cancer Res 12:322-34 (2014). Read more (PubMed: 24464915) »
  • Jin R  et al. Dietary fructose reduction improves markers of cardiovascular disease risk in Hispanic-American adolescents with NAFLD. Nutrients 6:3187-201 (2014). Human . Read more (PubMed: 25111123) »

See all 2 Publications for this product

Product Wall

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I can confirm that Heparin should not be interfering with the assay using these kits.

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I checked with the lab and they let me know that the homology with mouse is only 2%, thus they do not recommend using it for this species. It will most likely not work.

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Thank you for contacting Abcam regarding ab108891.


Our human kit is specific for human PAI-1 total antigen measurement. It does not cross react with rabbit PAI-1. Unfortunately, we do not have a rabbit specific kit available.

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Thank you for your enquiry.

This kit measures the total PAi-1 including free PAI-1, PAI-tPA complexes and PAI-1-uPA complexes.

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All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

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I am sorry about the confusion over the labeling of the standards for ab108891 and ab108914. I have heard back from the lab about the correct concentrations for these standards:

ab108891 - Recon...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"