Overview

  • Product nameHuman PAI1 + tPA ELISA Kit
    See all PAI1 + tPA kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Tissue
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.06 ng/ml
  • Range
    0.063 ng/ml - 4 ng/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PAI1 + tPA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 + tPA concentrations in plasma, serum, tissue extracts and cell culture supernatants.


    A PAI1 + tPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 + tPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 + tPA captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human PAI1+tPA ELISA Kit (ab192151) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Cellular localizationSecreted
  • Alternative names
    • Alteplase
    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI 1
    • PAI1
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpin E1
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • SERPINE1
    • T PA
    • T plasminogen activator
    • tissue plasminogen activator
    • Tissue type plasminogen activator
    • TPA
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human PAI1 + tPA ELISA Kit images

  • Representative Standard Curve using ab108892.

Protocols

References for Human PAI1 + tPA ELISA Kit (ab108892)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from this kit.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forELISA on human samp...

Read More

Thank you for contacting Abcam.
I have been in contact with the lab about the possibility of using ab108892 and ab46509 with samples from Cynomolgus Monkey. Unfortunately, the cross-reactivity is low so it will not work well for monkey samples. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"