Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence
    • Species
      Human

Specifications

Our Abpromise guarantee covers the use of ab51559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

    Western blot

  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.02% Sodium Azide
    Constituents: 0.1% BSA, PBS, pH 7.2

General Info

  • Alternative names
    • C-t-PAK2
    • CB422
    • EC 2.7.11.1
    • Gamma PAK
    • Gamma-PAK
    • hPAK65
    • Kinase
    • p21 (CDKN1A) activated kinase 2
    • p21 (CDKN1A)-activated kinase 2a
    • p21 activated kinase 2
    • p21 protein (Cdc42/Rac)-activated kinase 2
    • p21 protein Cdc42 Rac activated kinase 2
    • p21-activated kinase 2
    • p21-activated kinase, 65-KD
    • p21-activated protein kinase I
    • p21CDKN1A activated kinase 2
    • p27
    • p34
    • p58
    • p65PAK
    • PAK 2
    • PAK-2
    • PAK-2p34
    • Pak2
    • PAK2_HUMAN
    • PAK65
    • PAKgamma
    • S6 H4 kinase
    • S6/H4 kinase
    • Serine threonine protein kinase PAK 2
    • Serine/threonine protein kinase PAK 2
    see all
  • Function
    The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
  • Tissue specificity
    Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
    During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
    Ubiquitinated, leading to its proteasomal degradation.
    PAK-2p34 is myristoylated.
  • Cellular localization
    Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
  • Information by UniProt

References

ab51559 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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