Human pancreas tissue lysate - total protein (ab29816)

Overview

  • Product nameHuman pancreas tissue lysate - total protein
    See all Pancreas lysates
  • Description
    Human pancreas total protein lysate
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab29816 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Human pancreas tissue lysate - total protein (ab29816)

    ab29816 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    ab29821 Loading Buffer_(ab29821) current lot donor is 64 Years Old, Female.
    ab29816 Loading Buffer_(ab29816) current lot donor is 73 Years Old, Female.

    I hope this information is helpful to you. Please...

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    Thank you for contacting us.

    I am happy to refund the tissue lysate ab29816.

    Your credit note ID is 23323

    I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to i...

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    Thank you for contacting us. I am sorry to hear this pancreas lysate is not providing satisfactory results.

    The product issued should have been 100 µg at 3.75mg/ml therefore you should have received approx 26.6 ul. Was this the case or...

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    Thank you for contacting us.

    I believe loading 20ug of the tissue lysate into the well should give you good bands on your western blot. I would use it at the concentration at which it has been provided at.

    I hope this information ...

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    Thank you for your participation in Abcam's post complaint survey.

    Please let me know if there is anything else I can do for you, or if you have questions to any of our products (e.g. primary antibodies, lysates, secondary antibodies, kits, c...

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    Thank you for contacting us.

    Your credit note IDs are xxx and xxx.

    I am sorry that this lysatedid not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed...

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    Thank you for your reply and also for checking the lysate. This is really helpful.

    I am sorry to hear that the problem has not been resolved.
    Since you see no protein bands before transfer, it does not surprise me that you see none after...

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    Thank you for your patience.

    I have heard back from the lab who checked the lot you were using, and they let me know the following:

    We have not got any complaint about this lot GR26918-2 in the past.

    We are sendingthe gel...

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    Thank you for contacting us.
    I am sorry to hear about the problem the pancreas lysate is causing you.

    As we discussed over the phone, it would be worthwhile trying the following in order to determine the cause of the problem:

    ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"