Anti-Human Papillomavirus 16 (E7) antibody [TVG 701Y] (ab20191)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberTVG 701Y
  • MyelomaNS0/1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceE7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription
  • Cellular localizationNuclear
  • Alternative names
    • E7 antibody
    • HPV16 E7 antibody
    • Protein E7 antibody
    • Transforming protein antibody

References for Anti-Human Papillomavirus 16 (E7) antibody [TVG 701Y] (ab20191)

This product has been referenced in:
  • Harris M  et al. Combined treatment of the experimental human papilloma virus-16-positive cervical and head and neck cancers with cisplatin and radioimmunotherapy targeting viral E6 oncoprotein. Br J Cancer 108:859-65 (2013). Read more (PubMed: 23385729) »
  • Tommasino M  et al. HPV16 E7 protein associates with the protein kinase p33CDK2 and cyclin A. Oncogene 8:195-202 (1993). Read more (PubMed: 8380917) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

I can confirm that this antibody is still under production and we expect this to be available in mid march or early April 2013. Many thanks for having patience!

I hope this information is helpful to you. P...

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Thank you for contacting us and for your patience.

The supplying lab let us know the following:

We do not have a protocol sincewe are provided our antibodies by outside scientists and we tend to see only the information that is prov...

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Thank you for your enquiry and your interest in our products.

These two monoclonal antibodies recognize the same protein (Human Papillomavirus 16 (E7)) but are from different clones.

http://www.abcam.com/ab20191:

http://ww...

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Thank you for your enquiry. I am pleased to help answer your questions.

1. I have contacted the originator and regrettably they are not able to provide any further information on the immunogen on this occasion.We aim to provide as much infor...

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Thank you for your call today and for your questions about ab20191.

I have done a search on google scholar for the clone TVG 701Y, and there are several publications listed:

http://scholar.google.com/scholar?q=TVG+701Y&hl=en&...

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Thank you for contacting Abcam.   I have contacted the lab about ab20191, which is a Human Papillomavirus 16 (E7) antibody [TVG701Y] and can be used in flow cytometry and western blot. However the lab has told me that we do not know what the immunogen ...

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Please fin attached the certificate of compliance for ab20191. If there is anything else I can help you with, please let me know.

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab20191 in immunohistochemistry. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support but...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"