The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Par 6 partitioning defective 6 C elegans homolog alpha
Par 6 partitioning defective 6 homolog alpha
Par 6 partitioning defective 6 homolog alpha C elegans
par-6 family cell polarity regulator alpha
Partitioning defective 6 homolog alpha
partitioning-defective protein 6
partitioning-defective protein 6, C. elegans, homolog of, alpha
Tax interacting protein 40
Tax interaction protein 40
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
Belongs to the PAR6 family. Contains 1 OPR domain. Contains 1 PDZ (DHR) domain. Contains 1 pseudo-CRIB domain.
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases. The OPR domain mediates interactions with MAP2K5. The PDZ domain mediates the interaction with CRB3.
Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.