The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-PDE5A antibody (ab64179)
Purity70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase
cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase
cGMP binding cGMP specific phosphodiesterase
cGMP binding/cGMP specific phosphodiesterase
cGMP specific 3'5' cyclic phosphodiesterase
cGMP specific phosphodiesterase PDE5A2
cGMP specific phosphodiesterase type 5A
cGMP-binding cGMP-specific phosphodiesterase
Phosphodiesterase 5A cGMP specific
Phosphodiesterase isozyme 5
FunctionPlays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
Tissue specificityExpressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.