|Sample type||Average %||Range|
|Cell culture extracts||94.37||83% - 105%|
|Tissue Extracts||92.78||82% - 104%|
Abcam’s PDGF BB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF BB in cell lysates and tissue lysates.
This assay employs an antibody specific for Human PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2X Cell Lysate Buffer||1 x 5ml|
|5X Assay Diluent||1 x 15ml|
|5X Sample Diluent Buffer||1 x 10ml|
|800X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated anti-Human PDGF BB||2 vials|
|PDGF BB Microplate (12 x 8 wells)||1 unit|
|Recombinant Human PDGF BB Standard||2 x 1ml|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100625 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100625 has not yet been referenced specifically in any publications.