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|Sample type||Average %||Range|
|Serum||= 103.7||106% - 112%|
|Plasma||= 87.02||76% - 97%|
|Cell culture media||= 106.7||100% - 115%|
Abcam’s PDGF R-alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human PDGF R-alpha (CD140) in serum, plasma (Collect plasma using EDTA and heparin as anticoagulants. Citrate is not recommended as an anticoagulant for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human PDGF R-alpha (CD140) coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF R-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF R-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF R-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Human PDGF R-alpha||2 vials|
|PDGF R-alpha Microplate (12 x 8 wells)||1 unit|
|Recombinant Human PDGF R-alpha Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab155438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab155438 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"