Human PDGF Receptor beta ELISA Kit (ab100626)

Overview

  • Product nameHuman PDGF Receptor beta ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 25 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 90.39 76% - 104%
    Serum 109.6 97% - 118%
    Plasma 96.23 81% - 117%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PDGF Receptor beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF Receptor beta in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PDGF Receptor beta coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF Receptor beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF Receptor beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF Receptor beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionGrowth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • Tissue specificityExpressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • Cellular localizationSecreted. Released by platelets upon wounding.
  • Information by UniProt
  • Alternative names
    • Beta type platelet derived growth factor receptor
    • CD140 antigen like family member B
    • CD140b
    • JTK12
    • PDGF R beta
    • PDGF subunit B
    • PDGF-2
    • Pdgfb
    • PDGFB_HUMAN
    • PDGFR
    • PDGFR beta
    • PDGFR1
    • PDGFRB
    • Platelet derived growth factor receptor beta
    • Platelet derived growth factor receptor beta polypeptide
    • Platelet-derived growth factor B chain
    • Platelet-derived growth factor beta polypeptide
    • Platelet-derived growth factor subunit B
    • Proto-oncogene c-Sis
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human PDGF Receptor beta ELISA Kit images

  • Representative Standard Curve using ab100626.

Protocols

References for Human PDGF Receptor beta ELISA Kit (ab100626)

ab100626 has not yet been referenced specifically in any publications.

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