|Sample type||Average %||Range|
|Cell culture supernatant||71||% - %|
Abcam’s Perforin (PRF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Perforin in supernatant and buffered solutions.
A monoclonal antibody specific for Perforin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Perforin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Perforin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Perforin present in the samples.
This kit will recognize both endogenous and recombinant Human Perforin.
|Components||Identifier||1 x 96 tests||2 x 96 tests|
|1X Standard Diluent Buffer||1 x 25ml||2 x 25ml|
|200X Wash Buffer||White||1 x 10ml||2 x 10ml|
|Biotinylated anti PRF1||Red||1 x 0.4ml||2 x 0.4ml|
|Biotinylated Antibody Diluent||Red||1 x 7.5ml||1 x 13ml|
|Chromogen TMB Substrate Solution||1 x 11ml||1 x 24ml|
|HRP Diluent||Red||1 x 23ml||1 x 23ml|
|PRF1 Microplate (12 x 8 well strips)||1 unit||2 units|
|PRF1 Standard (Lyophilized)||Yellow||2 vials||4 vials|
|Stop Reagent||Black||1 x 11ml||2 x 11ml|
|Streptavidin HRP||2 x 5µl||4 x 5µl|
Our Abpromise guarantee covers the use of ab46068 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Perforin measured in cell culture supernatants (tested at 1/1-1/100 dilution range; duplicates +/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).
Perforin measured in biological fluids (dilution range 1/1-1/100; duplicates +/- SD).
Representative standard curve using ab46068