Abcam’s Perforin Human in vitro ELISA Set is designed for the quantitative measurement of Perforin in cell culture supernatants and buffered solutions.
The Perforin ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step. This ELISA set is intended to measure Perforin in cell -culture supernatants, as well as in serum samples*. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
*Special note for serum & plasma quantification: high and non linear detection level is found in human serum and plasma samples. The high signal appears not dependant to the antibody pairs but probably to the matrix and/ or perforin interaction with other molecules. Consequently serum and plasma quantification is under the scientist responsibility and specific investigations. Remark: no detection signal was found in fetal calf serum, rat, mouse and horse serum
Sensitivity: < 40 pg/ml
|Components||5 x 96 tests||10 x 96 tests|
|Detection biotinylated anti-Perforin antibody||1 x 0.55ml||2 x 0.55ml|
|Perforin Capture Antibody||1 x 0.5ml||2 x 0.5ml|
|Perforin standard||5 vials||10 vials|
|Ready-to-use TMB||2 vials||4 vials|
|Streptavidin-HRP||1 vial||2 vials|
Our Abpromise guarantee covers the use of ab83709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab83709 has not yet been referenced specifically in any publications.