Overview

  • Product name
    Human Periostin ELISA Kit (OSF2)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 16 1392pg/ml 90.48 6.5%
    2 16 2593pg/ml 121.9 4.7%
    3 16 4831pg/ml 280.2 5.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1436pg/ml 107.7 7.5%
    2 24 2734pg/ml 153 5.6%
    3 24 5166pg/ml 330.6 6.4%
  • Sample type
    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay time
    3h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human Periostin Enzyme-Linked Immunosorbent Assay (ELISA) kit (OSF2) (ab213816) is designed for the quantitative measurement of Human Periostin in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Periostin/OSF2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: N22-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin/OSF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Periostin/OSF2 amount of sample captured in plate.

  • Notes

    Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was over-expressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human Periostin ELISA Kit (OSF2) (ab213816) Standard Curve.

Protocols

References

ab213816 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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