Human peripheral blood leukocyte normal tissue lysate - total protein (ab29135)

Overview

  • Product nameHuman peripheral blood leukocyte normal tissue lysate - total protein
    See all Peripheral blood leukocyte lysates
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab29135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Human peripheral blood leukocyte normal tissue lysate - total protein (ab29135)

    ab29135 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email and the data. Yes, I agree that the problems seems to lie with the lysate and not so much with the antibody you are using. The antibody has been tested with human as well as mouse samples.

    I am issuing you the follow...

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    Thank you for the additional information on CD45 expression.

    Yes, I agree that CD45 should be present on the leukocytes, and potentially be detectable in the lysate ab29135.

    Please let me know if a replacement would be best in order...

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    I just received some additional information from the lab that might be of help:

    Actually I did a bit google search on CD45 expression in blood cell, it seems quite complicated, and there are several literatures indicating the CD45 expression ...

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    Thank you for your patience.

    I have heard back from the lab with the following information:

    We have checkedour product QC data, but we can't guarantee that there will be CD45 and Runx1 expression in the lysate, since they are not o...

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    Thank you for your time to participate in the recent survey. I am very sorry to hear that the replacement lysate also did not work. I called and left a message for you. I will try calling again, later in the day. Please let me know if the issues were t...

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    Thank you for email and the details provided which will enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued ...

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    Thank you for your enquiry. This product has not been boiled. Therefore, you will still need to boil the sample before loading on SDS-PAGE. If there is anything else that I can help you with, please do not hesitate to contact me.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"