Overview

  • Product nameHuman PF4 ELISA Kit (CXCL4)
    See all PF4 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    20.58 pg/ml - 15000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108.2 101% - 116%
    Serum 122.4 110% - 130%
    Plasma 119.6 111% - 127%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PF4 (CXCL4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PF4 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Save time with a 90-minute ELISA protocol. Try Human PF4 ELISA Kit (CXCL4) (ab189573) from our SimpleStep ELISA® range.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionReleased during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications
    Binds non-covalently to a proteoglycan molecule.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C-X-C motif chemokine 4
    • Chemokine (C X C motif) ligand 4
    • Chemokine (CXC motif) ligand 4
    • CXCL 4
    • CXCL4
    • Iroplact
    • MGC138298
    • Oncostatin A
    • Oncostatin-A
    • OncostatinA
    • PF 4
    • PF-4
    • PF4
    • Platelet factor 4
    • PLF4_HUMAN
    • SCYB 4
    • SCYB4
    • short form
    • Small inducible cytokine subfamily member 4
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab100628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human PF4 ELISA Kit (CXCL4) images

  • Human PF4 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 200-800 fold.

  • Representative Standard Curve using ab100628.

Protocols

References for Human PF4 ELISA Kit (CXCL4) (ab100628)

ab100628 has not yet been referenced specifically in any publications.

Product Wall

One of the two vials of the PF4 (CXCL4) standard should be reconstituted in 400 uL Assay diluent C (for serum/plasma samples) or 1X Assay Diluent B (for cell culture medium and urine). This will yield a 140 ng/ml stock standard solution. The second via...

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The 2nd PF4 Standard vial is just an extra vial. It is there to minimize freeze-thaw cycles in case you want to perform multiple experiments with the kit on different days.

Thank you for your inquiry regarding ab100628.

Unfortunately, we do not know whether this PF4 Human ELISA kit will also recognize the bound PF4-heparin complex form. All we know is that the antibody pair is binding on the PF4 protein somewher...

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Thank you for contacting us this afternoon and for your interest in Abcam.

Following on from our phone call I have discussed with my colleague xxxxx in marketing whether we have any start-up offers available. He informed me that this is someth...

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Thank you for your inquiry.

I have to confirm that we have not tested this kit with bone marrow aspirate. If the bone marrow aspirate cell plasma is prepared cell free as a clear liquid we believe could be suitable for this sample type. But t...

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This kit has been successfully tested and validated with EDTA, citrate, and heparin plasma. Below are some general tips for sample preparation that will hopefully be useful for you:



TIPS FOR SAMPLE PREPARATION

How do I ma...

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Thank you for contacting us. The lab let me know the following: Our recommended human serum dilution for ab100628 is 200-2,000 fold.  Please note that this recommendation should only be used as a starting point and the optimal dilution will need ...

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Désolé pour la confusion à propos des vitesses de centrifugation. La préparation des échantillons plasma ou sérum doit se faire à 3000g. Le nombre de rotation par minute sera déterminé en fonction de la taille du rotor de la centrifugeuse utilisée. J'e...

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Merci de nous avoir contactés. J'ai reçu les informations suivantes concernant les kits ELISA CD40L et PF4 : ab99991 doit être utilisé avec des échantillons de plasma traités EDTA ou héparine. L'utilisation d'autres anticoagulants n'est pas recomma...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"