Human Phospholipase A2 IIE full length protein (ab63845)

Overview

  • Product nameHuman Phospholipase A2 IIE full length protein

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence
    • SpeciesHuman
    • SequenceMRGSHHHHHH GMASHMNLVQ FGVMIEKMTG KSALQYNDYG CYCGIGGSHW PVDQTDWCCH AHDCCYGRLE KLGCEPKLEK YLFSVSERGI FCAGRTTCQR LTCECDKRAA LCFRRNLGTY NRKYAHYPNK LCTGPTPPC
    • Amino acids20 to 142

Specifications

Our Abpromise guarantee covers the use of ab63845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Purity> 95 % by SDS-PAGE.

  • FormLyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    0.05M Acetate buffer pH 4.0

  • ReconstitutionAdd 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

General info

  • Alternative names
    • 2IIE
    • EC 3.1.1.4
    • GIIE sPLA2
    • Group IIE secretory phospholipase A2
    • Group IIE secretory phospholipase A2 precursor
    • PA2GE_HUMAN
    • Phosphatidylcholine 2 acylhydrolase GIIE
    • Phosphatidylcholine 2-acylhydrolase 2E
    • Phospholipase A2 group IIE
    • Pla2g2e
    • sPLA
    • sPLA2-IIE
    see all
  • FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids.
  • Tissue specificityRestricted to the brain, heart, lung, and placenta.
  • Sequence similaritiesBelongs to the phospholipase A2 family.
  • Cellular localizationSecreted.
  • Information by UniProt

Human Phospholipase A2 IIE full length protein images

  • 12% SDS-PAGE separation of ab63845. Molecular Weight: ~15.8 kDa.

    Lane 1. Molecular weight marker
    Lane 2. reduced and heated sample, 5µg/lane
    Lane 3. non-reduced and non-heated sample, 5µg/lane

References for Human Phospholipase A2 IIE full length protein (ab63845)

ab63845 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"