Human placenta normal tissue lysate - membrane extract (ab29743)

Overview

  • Product nameHuman placenta normal tissue lysate - membrane extract
    See all Placenta lysates
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notesProtein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab29743 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for Human placenta normal tissue lysate - membrane extract (ab29743)

    ab29743 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for getting back to me promptly.

    It is important to note that the actual (apparent) molecular weight values for the prestained markers can be different from the non-stained markers and also in different gel types.

    When a p...

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    Thank you for getting back to me and for sharing your results with me.

    The storage buffer of the positive control lysate contains beta mercaptoethanol (reducing agent) so it is very unlikely that the protein is dimerized particularly if the W...

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    Thank you for your e-mail.

    I can confirm that the package has been delivered this morning 20Jul12 at 10:14 GMT and the person who signed for was: J. XXX.

    Please check your Goods In Department. For your reference, tracking number was...

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    Thank you for your new contact.

    As we discussed and agreed previously, I have placed the second free of charge replacement vial (ab29743) - exchange for the original product ab29745. The new order number is No: 1125597.

    Currently, w...

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    Just to confirm that you do not have to pay for the free of charge replacement vial. I have raised a credit note for the incorrect amount of £237.60 that has been charged. The reference number for the credit note is: CN21732

    Apologies! ...

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    Thank you for sharing your recent results and providing the WB images..

    My view is that you detect BCRP/ABCG2 in the positive control as well as in the samples.

    However, there are a few things to consider:

    There are some s...

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    Thank you for your e-mail.

    Currently, the vial is in transit and we are waiting to get it from our Boston office. Hopefully today or Tuesday we will be able to dispatch it for you.

    If you need any further assistance in the future, ple...

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    Thank you for your response.

    I appreciate the time you have spent on these experiments in the laboratory and it is disappointing the results have not been successful. Normally, we would suggest loading 20-25 ug total protein per lane.

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    Thank you for getting back to me promptly and for providing some further details.

    The protocol seems to be absolutely fine. Since you have applied a sensitive LICOR Odyssey IR Imaging System for detection, the concentrations of the primary and...

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    Thank you for your enquiry. It was very nice meeting you in Dundee.



    I am sorry to hear that you have been experiencing problems using this product (ab29745) in the application that you wish to use it.



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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"