Anti-Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396)

Overview

  • Product name
    Anti-Human placental lactogen (epitope 2) antibody [INN-hPL-37]
  • Description
    Mouse monoclonal [INN-hPL-37] to Human placental lactogen (epitope 2)
  • Specificity
    Radioimmunological direct binding studies were performed with 125I-labelled. hPL, hGH, hPRL and increasing amounts of INN-hPL-37. In addition, competitive RIA with unlabelled hGH, hPRL and hPL were used for characterization. In competitive RIA cross reactivity with hGH and hPRL was below 1%. Direct binding demonstrated 39% reactivity with hGH but no cross reactivity with hPRL.
  • Tested applications
    Suitable for: RIAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human placental lactogen.

  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purification by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    INN-hPL-37
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent dilution.

Target

  • Relevance
    Placental lactogen is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • choriomammotropin antibody
    • Chorionic somatomammotropin A antibody
    • Chorionic somatomammotropin hormone 1 (placental lactogen) antibody
    • chorionic somatomammotropin hormone 1 antibody
    • Chorionic somatomammotropin hormone 2 antibody
    • CS 1 antibody
    • CS-2 antibody
    • CS-B antibody
    • CSA antibody
    • CSB antibody
    • CSH1 antibody
    • CSMT antibody
    • hCSB antibody
    • Lactogen precursor antibody
    • Lactogen, placental antibody
    • PL antibody
    • placental lactogen antibody
    see all

Images

  • ab11396 at 1/100 staining human BeWo cells (placenta, choriocarcinoma) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary antibody. The image is shown in the upper right hand panel. In this experiment ab15554 was used as a positive control (bottom left hand panel). Cells were counter stained with DAPI (upper left hand panel).

    See Abreview

References

This product has been referenced in:
  • Jin F  et al. Lentivirus-mediated PHLDA2 overexpression inhibits trophoblast proliferation, migration and invasion, and induces apoptosis. Int J Mol Med 37:949-57 (2016). IF . Read more (PubMed: 26935516) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Human recombinant hPL)
Loading amount
0.1 µg
Specification
Human recombinant hPL
Blocking step
Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Stoil Dmitrov

Verified customer

Submitted Apr 16 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (BeWo, placenta, choriocarcinoma)
Specification
BeWo, placenta, choriocarcinoma
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Stoil Dmitrov

Verified customer

Submitted Apr 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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