|Sample type||Average %||Range|
|Saliva||128.1||118.4% - 139.8%|
|Urine||118.6||106.9% - 130.3%|
|Cell culture media||104.4||102% - 105.9%|
Abcam’s Plasminogen (PLG) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Plasminogen protein in human serum, plasma, saliva, urine and cell culture supernatant samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Plasminogen is a secreted protein involved in blood coagulation and is a zymogen of plasmin. Plasmin dissolves the fibrin of blood clots into soluble products and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, plasmin weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Plasmin cleavage of fibronectin and laminin leads to cell detachment and apoptosis. It also cleaves thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Plasmin preferentially cleave Lys-|-Xaa to Arg-|-Xaa with higher selectivity than trypsin. Plasmin binds to cells. Plasminogen is converted into plasmin by plasminogen activators, both plasminogen and its activator being bound to fibrin. It is activated with catalytic amounts of streptokinase. Plasmin activity inhibited by SERPINE2. Plasmin is inactivated by alpha-2-antiplasmin immediately after dissociation from the clot. Plasminogen can be also cleaved into Angiostatin. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo. Plasminogen deficiency (PLGD) [MIM:217090] is a disorder characterized by decreased serum plasminogen activity. Two forms of the disorder are distinguished: type 1 deficiency is additionally characterized by decreased plasminogen antigen levels and clinical symptoms, whereas type 2 deficiency, also known as dysplasminogenemia, is characterized by normal, or slightly reduced antigen levels, and absence of clinical manifestations. Plasminogen deficiency type 1 results in markedly impaired extracellular fibrinolysis and chronic mucosal pseudomembranous lesions due to subepithelial fibrin deposition and inflammation. The most common clinical manifestation of type 1 deficiency is ligneous conjunctivitis in which pseudomembranes formation on the palpebral surfaces of the eye progresses to white, yellow-white, or red thick masses with a wood-like consistency that replace the normal mucosa. The disease is caused by mutations affecting the gene represented in this entry.
|Components||1 x 96 tests|
|10X Human Plasminogen Capture Antibody||1 x 600µl|
|10X Human Plasminogen Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent CPI - HAMA Blocker (ab193969)||1 x 6ml|
|Human Plasminogen Lyophilized Purified Protein||2 vials|
|Plate Seals||1 unit|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Sample Diluent NS (ab193972)||1 x 50ml|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab196262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Background-subtracted data values (mean +/- SD) are graphed.
6,000X diluted serum samples from 10 apparently healthy male donors (1 – 10) were measured in triplicates using this kit. Interpolated data values corrected for sample dilution are graphed in µg of Plasminogen per mL of serum (mean +/- SD, n=3). The mean of Plasminogen concentration of these serum samples was determined to be 161.9 µg /mL with a range of 112.3 – 194.6 µg /mL.
Background-subtracted data values (mean +/- SD, n = 3) are graphed.
Serial dilutions of Human native purified angiostatin (ab88558) and Human native purified plasmin (ab90928) along with Plasminogen Human Native Protein standard and 26 Blank wells were analyzed. The Plasminogen standard curve was prepared as described in Section 10. Raw data values (mean +/- SD) are graphed. The Mean of Blank controls is indicated by dotted line. Using the Plasminogen standard curve, the MDD in this experiment was determined to be 156 pg/mL.
ab196262 has not yet been referenced specifically in any publications.