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Abcam’s Plasminogen (PLG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Plasminogen concentrations in Human plasma, serum, urine, saliva, milk, tissue samples, cerebrospinal fluid and cell culture supernatants.
A Plasminogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Plasminogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Plasminogen captured in plate.
Get higher sensitivity in only 90 minutes with Human Plasminogen ELISA Kit (ab196262) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent M Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Plasminogen Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Plasminogen Microplate (12 x 8 well strips)||1 unit|
|Plasminogen Standard||1 vial|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Plasminogen measured in biological fluids and cell culture supernatants showing quantity (ng) per mL of tested sample. Human serum and plasma samples diluted 20000-160000 fold. Other samples diluted 1-100 fold.