Overview

  • Product name
    Human PMN Elastase ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5.6%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.98 pg/ml
  • Range
    0.16 ng/ml - 10 ng/ml
  • Recovery

    104 %

    Sample specific recovery
    Sample type Average % Range
    Plasma 104 96% - 110%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.


    PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate. 


    The capture antibody in the Human PMN-Elastase ELISA kit is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • PMN elastase measured in the high control and low control (ab119553) and in biological fluids (dilution range 1/1-1/1000; duplicates +/- SD).

  • Representative Standard Curve using ab119553.

Protocols

References

This product has been referenced in:
  • Schechter MC  et al. Neutrophil extracellular trap (NET) levels in human plasma are associated with active TB. PLoS One 12:e0182587 (2017). ELISA ; Human . Read more (PubMed: 28777804) »
  • Bou Ghanem EN  et al. The Alpha-Tocopherol Form of Vitamin E Boosts Elastase Activity of Human PMNs and Their Ability to Kill Streptococcus pneumoniae. Front Cell Infect Microbiol 7:161 (2017). Read more (PubMed: 28516066) »

See all 5 Publications for this product

Customer reviews and Q&As

Fresh plasma samples are excellent ELISA samples however 1 time frozen and thawed should be fine as well. Generally, freeze thaw 3-4 times is very detrimental for plasma/serum proteins therefore we suggest avoiding multiple freeze thawing.
In case...

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The PMN elastase ELISA ab119553 has not, to our knowledge, been used to test sputum samples, but it will in theory be compatible with that sample type. The question is whether the assay will be sensitive enough to detect the amounts of elastase present...

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Elastase levels in gut mucosa lysates

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Followed the kit's protocol from start to finish.
The samples were whole tissue lysates of human gut mucosa which were assayed in various dilutions (in PBS). Have not tried any other sample type.
Kit is very easy to use and works just like any standard ELISA.
Got consitently good results. Will buy it again.
Username

Abcam user community

Verified customer

Submitted Mar 13 2014



This kit can be used with serum and plasma.


This kit works with plasma treated with EDTA or citrate.
We have no information pertaining to the use of other anti-coagulants in use with this kit.

I am sorry for the delay in getting back to you.

I now have further information to share with you in regards to the Neutrophil Elastase ELISA you are hoping to set up.

If you would be interested in setting up your own ELISA I have loo...

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Thank you for contacting us and your interest in our products.

I am sorry but having looked into it, we are unfortunately not able to provide the standard or high/low samples for the PMN Elastase Human ELISA kit (ab119553) separately.

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Thank you for contacting us.
Unfortunately, it is not possible to obtain the standard and detection antibody separately for this kit. Also, the coating antibody is bound on the plates and is not available separately either.
I am sorry to not ...

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Thank you for contacting Abcam regarding ab119553.


I can confirm that this kit will only recognize the complex of PMN elastase/alpha 1-PI complex standards and also our standard is lyophilized complex.


I hope this inform...

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Ihave issued a freekit, 8-hydroxy 2 deoxyguanosine ELISA Kit, ab101245,with the order number 1070428.

To check the status of the order please contact our Customer Service team and reference this number.

Again, I apologize for any ...

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1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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