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|Sample type||Average %||Range|
|Plasma||104||96% - 110%|
Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.
PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate.
The capture antibody in the Human PMN-Elastase Platinum ELISA,BMS269 ELISA is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.”
|Components||1 x 96 tests|
|Adhesive Films||2 units|
|Control high||1 vial|
|Control low||1 vial|
|HRP-Conjugate anti-alpha1-PI polyclonal antibody||1 x 16ml|
|Human PMN Elastase Standard, lyophilized, (10 ng/mL upon reconstitution)||1 vial|
|Microplate coated with polyclonal antibody to Human PMN Elastase (12 x 8 wells)||1 unit|
|Sample Diluent||1 x 50ml|
|Stop Solution||1 x 7ml|
|TMB Substrate Solution||1 x 22ml|
|Wash Buffer Concentrate (10x)||1 x 50ml|
Our Abpromise guarantee covers the use of ab119553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve with background signal subtracted (duplicates; +/- SD).
PMN elastase measured in the high control and low control (ab119553) and in biological fluids (dilution range 1/1-1/1000; duplicates +/- SD).
Representative Standard Curve using ab119553.