Anti-Human Polyoma virus JCV capsid protein VP1 antibody (ab34756)

Overview

  • Product nameAnti-Human Polyoma virus JCV capsid protein VP1 antibody
  • Description
    Mouse monoclonal to Human Polyoma virus JCV capsid protein VP1
  • SpecificityDoes not react with measles and mumps nucleocapsid proteins.
  • Tested applicationsSuitable for: Indirect ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with major capsid proteins VP1 of hamster polyomavirus (HaPyV) and SV40. It does not cross-react with major capsid protein VP1 human polyomavirus BKV and murine polyomavirus (MuPyV)
  • Immunogen

    Recombinant full length protein corresponding to Human Polyoma virus JCV capsid protein VP1. Recombinant full length purified major capsid protein VP1 of human polyomavirus JCV expressed in yeast S.cerevisiae.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA 1/1000 - 1/10000.
WB 1/1000 - 1/5000.
ICC/IF Use at an assay dependent concentration. PubMed: 25155602

Target

  • RelevanceThe human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins.

Anti-Human Polyoma virus JCV capsid protein VP1 antibody images

  • Immunoblot of recombinant major capsid protein VP1 (500 ng per lane) of human polyomavirus JCV using monoclonal antibody ab34756 at a concentration of 1 µg/ml.
  • ab34756 staining Human Polyoma virus JCV capsid protein VP1 in human fetal glial cell (SVG-A) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 2% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000 in PBS + BSA) for 1 hour at 37°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG (H+L) polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References for Anti-Human Polyoma virus JCV capsid protein VP1 antibody (ab34756)

This product has been referenced in:
  • Sharma BN  et al. Antiviral effects of artesunate on JC polyomavirus replication in COS-7 cells. Antimicrob Agents Chemother 58:6724-34 (2014). ICC/IF . Read more (PubMed: 25155602) »

See 1 Publication for this product

Product Wall

Thank you for your interest in Abcam.

We unfortunately do not sell this product, Human Polyoma virus JCV. This product however might be available from different companies. Please check it online. You can also try searching in bio compare websi...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Fetal glial cell (SVG-A))
Permeabilization No
Specification Fetal glial cell (SVG-A)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative Methanol
Username

Abcam user community

Verified customer

Submitted Jul 24 2015



Die Spezifitätsnotiz beschreibt folgendes:

ab34756 bindet das Major Capsidprotein VP1 von humanem Polyoma Virus JCV und das Capsidprotein VP1 von hamster polyomavirus (HaPyV) and Simian Virus 40 (SV40).

ab34756 kreuz-...

Read More

ab34756 recognizes the C-terminal part of human polyomavirus JCV capsid protein VP1. The epitope is localized between 290 and 384 aa residues of JCV capsid protein VP1.

DISCOUNT CODE: ***********
Expiration date: **************
I am very pleased to hear you would like to accept our offer and test ab34756 in IHC. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, ...

Read More

Thank you very much for your interest in ab34756.
To our knowledge, ab34756 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab34756 now, test it in IHC and submit feedback to us in the form of an Abre...

Read More

Thank you for your reply. I can confirm that the antibody ab34756 will react with the SV40 VP1 protein ab74565. On the antibody datasheet in the section labelled "cross-reactivity", it notes that it does react with the VP1 protein of SV40. I hope this...

Read More

Thank you for your call this week and for your patience while I have been in touch with the production lab about these proteins. I have confirmed with the lab that these proteins do not contain any tag. To detect them, you could use specific monoclonal...

Read More

DISCOUNT CODE: ****** Expiration date: February 11, 2012 I am very pleased to hear you would like to accept our offer and test ab34756 in ICC/IF. This code will give you one free primary antibody before the expiration date. To redeem this offer...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"