Human PreAlbumin ELISA Kit (Transthyretin) (ab108895)

Overview

  • Product nameHuman PreAlbumin ELISA Kit (Transthyretin)
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.08 ng/ml
  • Range
    0.08 ng/ml - 31.25 ng/ml
  • Recovery

    = 96 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PreAlbumin (Transthyretin) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PreAlbumin concentrations in plasma, serum, urine, saliva, milk and cell culture supernatants.

    A PreAlbumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PreAlbumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PreAlbumin captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionThyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • Tissue specificityDetected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
  • Involvement in diseaseDefects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR) [MIM:105210]. A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor.
    Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) [MIM:145680]. It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities.
    Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1) [MIM:115430]. It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis.
  • Sequence similaritiesBelongs to the transthyretin family.
  • DomainEach monomer has two 4-stranded beta sheets and the shape of a prolate ellipsoid. Antiparallel beta-sheet interactions link monomers into dimers. A short loop from each monomer forms the main dimer-dimer interaction. These two pairs of loops separate the opposed, convex beta-sheets of the dimers to form an internal channel.
  • Cellular localizationSecreted. Cytoplasm.
  • Information by UniProt
  • Alternative names
    • Amyloid polyneuropathy
    • Amyloidosis I
    • ATTR
    • Carpal tunnel syndrome 1
    • CTS
    • CTS1
    • Dysprealbuminemic euthyroidal hyperthyroxinemia
    • Dystransthyretinemic hyperthyroxinemia
    • Epididymis luminal protein 111
    • HEL111
    • HsT2651
    • PALB
    • Prealbumin
    • Prealbumin amyloidosis type I
    • Prealbumin Thyroxine-binding
    • Senile systemic amyloidosis
    • TBPA
    • Thyroxine binding prealbumin
    • Transthyretin
    • TTHY_HUMAN
    • TTR
    • TTR protein
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human PreAlbumin ELISA Kit (Transthyretin) images

  • Standard curve of Hu PreAlbumin, with background signal subtracted (duplicates; +/- SD).
  • PreAlbumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
  • PreAlbumin detected in cell supernatants, results shown after background signal was subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108895.

Protocols

References for Human PreAlbumin ELISA Kit (Transthyretin) (ab108895)

ab108895 has not yet been referenced specifically in any publications.

Product Wall

The 10x diluent can have usual particulate that forms. These are simple salt deposits that the process of diluting breaks up and dissolves and there is no worry that this will affect results from the kit as you have also seen in your experiments.
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You should use the protocol supplied in the box with the kit as the method is optimized for that batch of kit components.
In this case the difference relates mainly to extra information supplied with lot 123855 and minor differences in incubation ...

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Our source for this assay claims "the kit will measure total prealbumin level" and that "it can recognize all forms of prealbumin".

Thank you very much for contacting us with your question, and for your patience while I have been in touch with the lab regarding your enquiry.

This kit has been tested with samples from many different species, including bovine, monkey, mouse...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"