The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Heterogeneous nuclear ribonucleoprotein methyltransferase 1 like 2
Heterogeneous nuclear ribonucleoproteins methyltransferase like 2
HMT1 (hnRNP methyltransferase S. cerevisiae) like 2
HMT1 hnRNP methyltransferase
HMT1 hnRNP methyltransferase like 2
HMT1 hnRNP methyltransferase like 2 (S. cerevisiae)
Human mRNA for suppressor for yeast mutant
Human mRNA for suppressor for yeast mutant complete cds
Interferon receptor 1 bound protein 4
Interferon receptor 1 bound protein4
Interferon receptor 1-bound protein 4
Interferon receptor 1bound protein 4
Protein arginine methyltransferase 1
Protein arginine N methyltransferase 1
Protein arginine N methyltransferase1
Protein arginine N-methyltransferase 1
S. cerevisiae like 2
Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway.
Belongs to the protein arginine N-methyltransferase family.