Overview

  • Product name
    Human Progesterone ELISA Kit
    See all Progesterone kits
  • Detection method
    Colorimetric
  • Sample type
    Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    0.05 ng/ml
  • Range
    0.2 ng/ml - 40 ng/ml
  • Recovery

    106 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    Abcam’s Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Progesterone in serum and plasma. 

    A 96-well plate has been precoated with anti-Progesterone. Samples and the Progesterone-HRP conjugate are added to the wells, where Progesterone in the sample competes with the added Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Progesterone in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

Protocols

References

This product has been referenced in:
  • Zhu J  et al. Maternal N-Carbamylglutamate Supplementation during Early Pregnancy Enhances Embryonic Survival and Development through Modulation of the Endometrial Proteome in Gilts. J Nutr 145:2212-20 (2015). Competitive ELISA . Read more (PubMed: 26290006) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your inquiry.

The antibody coated on the plate is a polyclonal rabbit antiserum. Although we do not have experience, it should be able to detect progesterone in rats. However, we wouldn't be able to guarantee it since we haven't...

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Thank you very much for your inquiry.

We do have no ELISA kit against LH, FSH or Progesterone which are tested against the rat proteins. We do have however such kits tested against the human protein, for which we have however not yet tested w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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