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Abcam’s Prolactin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.
A 96-well plate has been precoated with anti-Prolactin IgG antibodies. Samples and standards and are added to the wells, where Prolactin in the sample and standards binds to the precoated antibody. Added Anti-Prolactin-HRP conjugate binds to the antibody-Prolactin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Prolactin in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|Anti-Prolactin Coated Microplate (12 x 8 wells)||1 unit|
|Anti-Prolactin HRP Conjugate||1 x 13ml|
|Prolactin Standard 0 - 0 ng/mL (Lyophilized)||1 vial|
|Prolactin Standard 1 - 5 ng/mL (Lyophilized)||1 vial|
|Prolactin Standard 2 - 15 ng/mL (Lyophilized)||1 vial|
|Prolactin Standard 3 - 50 ng/mL (Lyophilized)||1 vial|
|Prolactin Standard 4 - 100 ng/mL (Lyophilized)||1 vial|
|Prolactin Standard 5 - 200 ng/mL (Lyophilized)||1 vial|
|Stop Solution||1 x 11ml|
|TMB Substrate Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab108655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab108655 has not yet been referenced specifically in any publications.