Overview

  • Product nameHuman Prolactin ELISA Kit
    See all Prolactin kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Level 1 20 6.32 0.4 6.3%
    Level 2 20 19.22 1.19 6.18%
    Level 3 20 54.52 2.64 4.84%
    Inter-assay
    Sample n Mean SD CV%
    Level 1 20 6.58 0.45 6.77%
    Level 2 20 21.34 1.3 6.11%
    Level 3 20 58.39 2.93 5.01%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.25 ng/ml
  • Range
    5 ng/ml - 100 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.

    Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceVarious hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
  • Cellular localizationSecreted
  • Alternative names
    • AV290867
    • Lactogenic Hormone
    • Luteotropic Hormone
    • Mammotropin
    • OTTMUSP00000000651
    • PRL
    • Prl1a1
    • Prol
    • Prolactin precursor
    • RATPRLSD1
    • RNPROL
    • RP23-351I6.2
    • RP3-404K8.1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab108679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Protocols

References for Human Prolactin ELISA Kit (ab108679)

ab108679 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab108679.


I have confirmed that both antibodies in this kit are derived from Mouse.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"