Abcam’s Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.
Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
|Components||Identifier||1 x 96 tests|
|10X Wash Solution||1 x 50ml|
|Anti-Prolactin-HRP conjugate||1 x 12ml|
|Cover foil||1 unit|
|Prolactin control||1 x 1ml|
|Prolactin Standard 0: 0 ng/ml||1 x 1ml|
|Prolactin Standard 1: 5 ng/ml||1 x 1ml|
|Prolactin Standard 2: 10 ng/ml||1 x 1ml|
|Prolactin Standard 3: 25 ng/ml||1 x 1ml|
|Prolactin Standard 4: 50 ng/ml||1 x 1ml|
|Prolactin Standard 5: 100 ng/ml||1 x 1ml|
|Stop Solution||1 x 15ml|
|Streptavidin Coated Microplate (12 x 8 wells)||12 Strips of 8 wells||1 unit|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
ab108679 has not yet been referenced specifically in any publications.