Overview

  • Product name
    Human Prorenin ELISA Kit
    See all Prorenin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 20 0.126ng/ml 0.009 7.28%
    2 20 0.523ng/ml 0.028 4.88%
    3 20 5.12ng/ml 0.243 4.74%
    Inter-assay
    Sample n Mean SD CV%
    1 10 0.165ng/ml 0.016 9.7%
    2 10 0.532ng/ml 0.031 5.85%
    3 10 5.03ng/ml 0.203 4.04%
  • Sample type
    Urine, Tissue Extracts, Cell culture media
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.016 ng/ml
  • Range
    0.02 ng/ml - 10 ng/ml
  • Recovery

    108.75 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture media 108.75 98% - 132%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Prorenin ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative determination of Prorenin in non-plasma samples including cell culture media, tissue extracts, or urine samples. Active renin will not be detected by this assay. Prorenin is measured directly by ELISA without pretreatment of samples or conversion to renin. 

    Human Prorenin will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, anti-Human Prorenin primary antibody binds to the captured protein. Only Prorenin and not active renin will be detected by the primary antibody. Excess antibody is washed away and bound primary antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of Prorenin. The amount of color development is directly proportional to the concentration of Prorenin in the sample.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab169738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab169738 has not yet been referenced specifically in any publications.

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