Human Prostate Specific Antigen ELISA Kit (f-PSA) (ab108656)

Overview

  • Product nameHuman Prostate Specific Antigen ELISA Kit (f-PSA)
    See all Prostate Specific Antigen kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 0.1 ng/ml
  • Range
    0.1 ng/ml - 25 ng/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    For quantification down to 4.8 pg/mL in a 90 minute, no-wash ELISA assay, try Human Free Prostate Specific Antigen ELISA kit SimpleStep (ab188389).


    ab108656 is intended for the quantitative determination of the cancer antigen Prostate Specific Antigen (f-PSA) concentration in Human serum.


    Human Prostate Specific Antigen is a 33 kD serineproteinase which, in Human serum, is predominantly bound to alpha 1-antichymotrypsin (PSA-ACT) and alpha 2-macroglobulin (PSA-AMG). Trace amounts of alpha 1-antitrypsin and inter-alpha trypsin inhibitor bound to PSA can also be found. Any remaining PSA is in the free form (f-PSA). Current methods of screening men for prostate cancer utilize the detection of the major PSA-ACT form. Levels of 4.0 ng/ml or higher are strong indicators of the possibility of prostatic cancer. However, elevated serum PSA levels have also been attributed to benign prostatic hyperplasia and prostatitis, leading to a large percentage of false positive screening results. A potential solution to this problem involves the determination of free PSA levels. Preliminary studies have suggested that the percentage of free PSA is lower in patients with prostate cancer than those with benign prostatic hyperplasia. Thus, the measurement of free serum PSA in conjunction with total PSA, can improve specificity of prostate cancer screening in selected men with elevated total serum PSA levels, which would subsequently reduce unnecessary prostate biopsies with minimal effects on cancer detection rates.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • antigen, prostate-specific
    • APS
    • Gamma seminoprotein
    • Gamma-seminoprotein
    • hK3
    • Kallikrein 3
    • Kallikrein related peptidase 3
    • Kallikrein-3
    • KLK 3
    • KLK2A1
    • Klk3
    • KLK3_HUMAN
    • P-30 antigen
    • P30 antigen
    • Prostate-specific antigen
    • Psa
    • Semenogelase
    • Seminin
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Human Prostate Specific Antigen ELISA Kit (f-PSA) images

  • Human Prostate Specific Antigen (f-PSA) Standard Curve

Protocols

References for Human Prostate Specific Antigen ELISA Kit (f-PSA) (ab108656)

ab108656 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"