• Product nameHuman PSA ELISA Kit (KLK3)
    See all Prostate Specific Antigen kits
  • Detection methodColorimetric
  • Precision
    Sample n Mean SD CV%
    Overall < 10%
    Sample n Mean SD CV%
    Overall < 12%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    10.24 pg/ml - 2500 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 81% - 108%
    Serum 105.4 94% - 112%
    Plasma 80.63 72% - 94%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    For quantification down to 4.8 pg/mL in a 90 minute, no-wash ELISA assay, try Human Free Prostate Specific Antigen ELISA kit SimpleStep (ab188389).

    Abcam’s PSA Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PSA in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human PSA coated on a 96-well plate. Standards and samples are pipetted into the wells and PSA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PSA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PSA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • antigen, prostate-specific
    • APS
    • Gamma seminoprotein
    • Gamma-seminoprotein
    • hK3
    • Kallikrein 3
    • Kallikrein related peptidase 3
    • Kallikrein-3
    • KLK 3
    • KLK2A1
    • Klk3
    • KLK3_HUMAN
    • P-30 antigen
    • P30 antigen
    • Prostate-specific antigen
    • Psa
    • Semenogelase
    • Seminin
    see all
  • Database links


Our Abpromise guarantee covers the use of ab113327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human PSA ELISA Kit (KLK3) images

  • PSA (KLK3) measured in various biological fluids showing quantity (pg) per mL of sample tested

  • Representative standard curve using ab113327

  • Representative standard curve using ab113327


References for Human PSA ELISA Kit (KLK3) (ab113327)

This product has been referenced in:
  • Banville N  et al. A quantitative proteomic approach to identify significantly altered protein networks in the serum of patients with lymphangioleiomyomatosis (LAM). PLoS One 9:e105365 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25133674) »

See 1 Publication for this product

Product Wall

Assay diluent A is is only required for the preparation of the standards and samples, if your samples are plasma. Once the PSA is captured on the plate and the samples and standard solutions are discarded from the wells, the remainder of the assay does...

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Thank you for contacting us. Your credit note ID is . I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing de...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to off...

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Thank you for your response.

I am sorry that the initial suggestion that I was able to give and that these further suggestions did not improve blank reading and thereby the detection limits of this for you. Would you be willing to send me th...

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Thank you for contacting Abcam regarding your higher 0 reading.

I have had a chance to speak with the lab regarding this. Their reply follows:

In general, a 0 standard OD reading around 0.2 OD is not considered very high but is in...

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Code de remise : XX
Date d’expiration : 09. Juillet 2012

Je suis ravie que vous souhaitiez participer a notre offre et tester ab113327 chez le macaque. Le code de réduction correspondant est XX. Ce code de réduction est utilisable sous ...

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Thank you for your inquiry. Although our production team does not’t routinely test urine samples,we know this kit is suitable for urine samples (based on our past experience with it). This is a general guideline on preparation of urine for ELIS...

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